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mcherry genes  (Addgene inc)


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    Structured Review

    Addgene inc mcherry genes
    Mcherry Genes, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 2984 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/px330+mcherry+plasmid/pX330-U6-Chimeric_BB-CBh-hSpCas9+(Plasmid+%2342230)/pmc12796744-275-16-18
    Average 96 stars, based on 2984 article reviews
    mcherry genes - by Bioz Stars, 2026-09
    96/100 stars

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    Related Articles

    Clone Assay:

    Article Title: Gastrula-Premarked Posterior Enhancer Primes Posterior Tissue Development Through Cross-Talk with TGF-β Signaling Pathway.
    Article Snippet: .. [61] Briefly, small guided RNAs (sgRNAs) were designed according to the instruction fromChopchop website (http://chopchop.cbu.uib.no/). sgRNAs were annealed and cloned into linearized px330-mCherry plasmid (Addgene, Plasmid #98 750). .. Px330-mCherry-sgRNA was transfected into E14TG2a by Lipofectamine 3000 Transfection Reagent (Invitrogen, L3000008) following the manufacturer’s instructions.

    Article Title: Chromatin activity of IκBα mediates the exit from naïve pluripotency
    Article Snippet: .. After annealing, one of the gRNAs was cloned into SpCas9(BB)–2A-GFP (px458) plasmid (Addgene; Cat #48138), and the other gRNA was cloned into the px330-mCherry plasmid (modified from Addgene; Cat #98750 to incorporate mCherry reporter). mESCs were co-transfected with the two plasmids (px458-gRNA1 and px330-mCherry-gRNA2). ..

    Plasmid Preparation:

    Article Title: Gastrula-Premarked Posterior Enhancer Primes Posterior Tissue Development Through Cross-Talk with TGF-β Signaling Pathway.
    Article Snippet: .. [61] Briefly, small guided RNAs (sgRNAs) were designed according to the instruction fromChopchop website (http://chopchop.cbu.uib.no/). sgRNAs were annealed and cloned into linearized px330-mCherry plasmid (Addgene, Plasmid #98 750). .. Px330-mCherry-sgRNA was transfected into E14TG2a by Lipofectamine 3000 Transfection Reagent (Invitrogen, L3000008) following the manufacturer’s instructions.

    Article Title: Chromatin activity of IκBα mediates the exit from naïve pluripotency
    Article Snippet: .. After annealing, one of the gRNAs was cloned into SpCas9(BB)–2A-GFP (px458) plasmid (Addgene; Cat #48138), and the other gRNA was cloned into the px330-mCherry plasmid (modified from Addgene; Cat #98750 to incorporate mCherry reporter). mESCs were co-transfected with the two plasmids (px458-gRNA1 and px330-mCherry-gRNA2). ..

    Article Title: Positional BMP signaling orchestrates villus length in the small intestine
    Article Snippet: .. Plasmid pX330-mCherry-Rosa26-SG was constructed by inserting the sgRNA sequence of the Rosa26 gene trap range into the pX330-mCherry plasmid (Addgene, Plasmid# 98750) containing the sgRNA scaffold and Cas9 DNA sequence. .. To obtain the donor plasmid pT-4xBRE-miniP-tdTomato-PEST, the sequence containing pT-4xBRE-miniP-tdTomato-PEST and the homologous arm was ligated into pMD19T-Vector. pX330-mCherry-Rosa26-SG and pT-4xBRE-miniP-tdTomato-PEST were transfected to DKO-AG-haESCs by lipo2000 (Invitrogen, Cat#11668019).

    Article Title: Tmem45b modulates itch via endoplasmic reticulum calcium regulation
    Article Snippet: .. To generate CRISPR-Cas9 plasmid for Cre Knockin (KI), sgRNAs of C-terminal of target gene Mrgprd (GGTCTGAAGGGAGCCCAACC) were synthesized, annealed, and ligated to the pX330-mCherry plasmid (Addgene, #98750) which was digested with Bbs I (Thermo). .. For the construction of Cre KI DNA donor, the sequences encoding left homologous arm, T2A-SV40 NLS-Cre and right homologous arm were amplified and ligated to the linear pMD19T vector with 20 bp overlap in order by Seamless Cloning Kit (Beyotime, D7010S).

    Article Title: Mutation-Agnostic Base Editing of the Progerin Farnesylation Site Rescues Hutchinson-Gilford Progeria Syndrome Phenotypes in Neuromuscular Organoids
    Article Snippet: Cells were passaged approximately every four days using Versene solution (Thermo Fisher Scientific). .. To generate FATE-iPSCs, the px330-mCherry plasmid (Addgene #98750, a gift from Dr. Jinsong Li) was modified by replacing the Cas9 coding sequence with the SpRY-ABE8e variant from pCMV-SpRY-ABE8e (Addgene #185671, a gift from Dr. David Liu) using Gibson assembly. ..

    Article Title: Tmem45b modulates itch via endoplasmic reticulum calcium regulation.
    Article Snippet: .. To generate CRISPRCas9 plasmid for Cre Knockin (KI), sgRNAs of C-terminal of target gene Mrgprd (GGTCTGAAGGGAGCCCAACC) were synthesized, annealed, and ligated to the pX330-mCherry plasmid (Addgene, #98750) which was digested with Bbs I (Thermo). .. For the construction of Cre KI DNA donor, the sequences encoding left homologous arm, T2A-SV40 NLS-Cre and right homologous arm were amplified and ligated to the linear pMD19T vector with 20 bp overlap in order by Seamless Cloning Kit (Beyotime, D7010S).

    Article Title: Positional BMP signaling orchestrates villus length in the small intestine.
    Article Snippet: .. Plasmid pX330-mCherry-Rosa26-SG was constructed by inserting the sgRNA sequence of the Rosa26 gene trap range into the pX330-mCherry plasmid (Addgene, Plasmid#98750) containing the sgRNAscaffold and Cas9 DNA sequence. .. To obtain the donor plasmid pT-4xBRE-miniPtdTomato-PEST, the sequence containing pT-4xBRE-miniP-tdTomatoPEST and the homologous arm was ligated into pMD19T-Vector. pX330-mCherry-Rosa26-SG and pT-4xBRE-miniP-tdTomato-PEST were transfected to DKO-AG-haESCs by lipo2000 (Invitrogen, Cat#11668019).

    Modification:

    Article Title: Chromatin activity of IκBα mediates the exit from naïve pluripotency
    Article Snippet: .. After annealing, one of the gRNAs was cloned into SpCas9(BB)–2A-GFP (px458) plasmid (Addgene; Cat #48138), and the other gRNA was cloned into the px330-mCherry plasmid (modified from Addgene; Cat #98750 to incorporate mCherry reporter). mESCs were co-transfected with the two plasmids (px458-gRNA1 and px330-mCherry-gRNA2). ..

    Article Title: Mutation-Agnostic Base Editing of the Progerin Farnesylation Site Rescues Hutchinson-Gilford Progeria Syndrome Phenotypes in Neuromuscular Organoids
    Article Snippet: Cells were passaged approximately every four days using Versene solution (Thermo Fisher Scientific). .. To generate FATE-iPSCs, the px330-mCherry plasmid (Addgene #98750, a gift from Dr. Jinsong Li) was modified by replacing the Cas9 coding sequence with the SpRY-ABE8e variant from pCMV-SpRY-ABE8e (Addgene #185671, a gift from Dr. David Liu) using Gibson assembly. ..

    Construct:

    Article Title: Positional BMP signaling orchestrates villus length in the small intestine
    Article Snippet: .. Plasmid pX330-mCherry-Rosa26-SG was constructed by inserting the sgRNA sequence of the Rosa26 gene trap range into the pX330-mCherry plasmid (Addgene, Plasmid# 98750) containing the sgRNA scaffold and Cas9 DNA sequence. .. To obtain the donor plasmid pT-4xBRE-miniP-tdTomato-PEST, the sequence containing pT-4xBRE-miniP-tdTomato-PEST and the homologous arm was ligated into pMD19T-Vector. pX330-mCherry-Rosa26-SG and pT-4xBRE-miniP-tdTomato-PEST were transfected to DKO-AG-haESCs by lipo2000 (Invitrogen, Cat#11668019).

    Article Title: Positional BMP signaling orchestrates villus length in the small intestine.
    Article Snippet: .. Plasmid pX330-mCherry-Rosa26-SG was constructed by inserting the sgRNA sequence of the Rosa26 gene trap range into the pX330-mCherry plasmid (Addgene, Plasmid#98750) containing the sgRNAscaffold and Cas9 DNA sequence. .. To obtain the donor plasmid pT-4xBRE-miniPtdTomato-PEST, the sequence containing pT-4xBRE-miniP-tdTomatoPEST and the homologous arm was ligated into pMD19T-Vector. pX330-mCherry-Rosa26-SG and pT-4xBRE-miniP-tdTomato-PEST were transfected to DKO-AG-haESCs by lipo2000 (Invitrogen, Cat#11668019).

    Sequencing:

    Article Title: Positional BMP signaling orchestrates villus length in the small intestine
    Article Snippet: .. Plasmid pX330-mCherry-Rosa26-SG was constructed by inserting the sgRNA sequence of the Rosa26 gene trap range into the pX330-mCherry plasmid (Addgene, Plasmid# 98750) containing the sgRNA scaffold and Cas9 DNA sequence. .. To obtain the donor plasmid pT-4xBRE-miniP-tdTomato-PEST, the sequence containing pT-4xBRE-miniP-tdTomato-PEST and the homologous arm was ligated into pMD19T-Vector. pX330-mCherry-Rosa26-SG and pT-4xBRE-miniP-tdTomato-PEST were transfected to DKO-AG-haESCs by lipo2000 (Invitrogen, Cat#11668019).

    Article Title: Mutation-Agnostic Base Editing of the Progerin Farnesylation Site Rescues Hutchinson-Gilford Progeria Syndrome Phenotypes in Neuromuscular Organoids
    Article Snippet: Cells were passaged approximately every four days using Versene solution (Thermo Fisher Scientific). .. To generate FATE-iPSCs, the px330-mCherry plasmid (Addgene #98750, a gift from Dr. Jinsong Li) was modified by replacing the Cas9 coding sequence with the SpRY-ABE8e variant from pCMV-SpRY-ABE8e (Addgene #185671, a gift from Dr. David Liu) using Gibson assembly. ..

    Article Title: Positional BMP signaling orchestrates villus length in the small intestine.
    Article Snippet: .. Plasmid pX330-mCherry-Rosa26-SG was constructed by inserting the sgRNA sequence of the Rosa26 gene trap range into the pX330-mCherry plasmid (Addgene, Plasmid#98750) containing the sgRNAscaffold and Cas9 DNA sequence. .. To obtain the donor plasmid pT-4xBRE-miniPtdTomato-PEST, the sequence containing pT-4xBRE-miniP-tdTomatoPEST and the homologous arm was ligated into pMD19T-Vector. pX330-mCherry-Rosa26-SG and pT-4xBRE-miniP-tdTomato-PEST were transfected to DKO-AG-haESCs by lipo2000 (Invitrogen, Cat#11668019).

    other:

    Article Title: SPNS1 variants cause multiorgan disease and implicate lysophospholipid transport as critical for mTOR-regulated lipid homeostasis
    Article Snippet: Cells were lysed in RIPA (Thermo Fisher) supplemented with cOmplete protease inhibitor.

    CRISPR:

    Article Title: Tmem45b modulates itch via endoplasmic reticulum calcium regulation
    Article Snippet: .. To generate CRISPR-Cas9 plasmid for Cre Knockin (KI), sgRNAs of C-terminal of target gene Mrgprd (GGTCTGAAGGGAGCCCAACC) were synthesized, annealed, and ligated to the pX330-mCherry plasmid (Addgene, #98750) which was digested with Bbs I (Thermo). .. For the construction of Cre KI DNA donor, the sequences encoding left homologous arm, T2A-SV40 NLS-Cre and right homologous arm were amplified and ligated to the linear pMD19T vector with 20 bp overlap in order by Seamless Cloning Kit (Beyotime, D7010S).

    Knock-In:

    Article Title: Tmem45b modulates itch via endoplasmic reticulum calcium regulation
    Article Snippet: .. To generate CRISPR-Cas9 plasmid for Cre Knockin (KI), sgRNAs of C-terminal of target gene Mrgprd (GGTCTGAAGGGAGCCCAACC) were synthesized, annealed, and ligated to the pX330-mCherry plasmid (Addgene, #98750) which was digested with Bbs I (Thermo). .. For the construction of Cre KI DNA donor, the sequences encoding left homologous arm, T2A-SV40 NLS-Cre and right homologous arm were amplified and ligated to the linear pMD19T vector with 20 bp overlap in order by Seamless Cloning Kit (Beyotime, D7010S).

    Article Title: Tmem45b modulates itch via endoplasmic reticulum calcium regulation.
    Article Snippet: .. To generate CRISPRCas9 plasmid for Cre Knockin (KI), sgRNAs of C-terminal of target gene Mrgprd (GGTCTGAAGGGAGCCCAACC) were synthesized, annealed, and ligated to the pX330-mCherry plasmid (Addgene, #98750) which was digested with Bbs I (Thermo). .. For the construction of Cre KI DNA donor, the sequences encoding left homologous arm, T2A-SV40 NLS-Cre and right homologous arm were amplified and ligated to the linear pMD19T vector with 20 bp overlap in order by Seamless Cloning Kit (Beyotime, D7010S).

    Synthesized:

    Article Title: Tmem45b modulates itch via endoplasmic reticulum calcium regulation
    Article Snippet: .. To generate CRISPR-Cas9 plasmid for Cre Knockin (KI), sgRNAs of C-terminal of target gene Mrgprd (GGTCTGAAGGGAGCCCAACC) were synthesized, annealed, and ligated to the pX330-mCherry plasmid (Addgene, #98750) which was digested with Bbs I (Thermo). .. For the construction of Cre KI DNA donor, the sequences encoding left homologous arm, T2A-SV40 NLS-Cre and right homologous arm were amplified and ligated to the linear pMD19T vector with 20 bp overlap in order by Seamless Cloning Kit (Beyotime, D7010S).

    Article Title: Tmem45b modulates itch via endoplasmic reticulum calcium regulation.
    Article Snippet: .. To generate CRISPRCas9 plasmid for Cre Knockin (KI), sgRNAs of C-terminal of target gene Mrgprd (GGTCTGAAGGGAGCCCAACC) were synthesized, annealed, and ligated to the pX330-mCherry plasmid (Addgene, #98750) which was digested with Bbs I (Thermo). .. For the construction of Cre KI DNA donor, the sequences encoding left homologous arm, T2A-SV40 NLS-Cre and right homologous arm were amplified and ligated to the linear pMD19T vector with 20 bp overlap in order by Seamless Cloning Kit (Beyotime, D7010S).

    Variant Assay:

    Article Title: Mutation-Agnostic Base Editing of the Progerin Farnesylation Site Rescues Hutchinson-Gilford Progeria Syndrome Phenotypes in Neuromuscular Organoids
    Article Snippet: Cells were passaged approximately every four days using Versene solution (Thermo Fisher Scientific). .. To generate FATE-iPSCs, the px330-mCherry plasmid (Addgene #98750, a gift from Dr. Jinsong Li) was modified by replacing the Cas9 coding sequence with the SpRY-ABE8e variant from pCMV-SpRY-ABE8e (Addgene #185671, a gift from Dr. David Liu) using Gibson assembly. ..



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